Coding

Part:BBa_K5048040:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


T7 promoter(lac repressible)-lasR-Plas-deGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 351
    Illegal AgeI site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this part in order to reproduce the result of the literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.



Source

complete synthesis

References